Calculate the final concentration when reconstituting lyophilized research peptides for in vitro assays, cell culture, and biochemical research.
For laboratory research only. This calculator is a tool for researchers preparing peptides for in vitro experiments, cell culture, and biochemical assays. Peptides sold by MySpaLive are not for human consumption, diagnosis, or treatment, and have not been evaluated by the FDA.
Enter your values to calculate concentration
Mass of lyophilized peptide in the vial
Volume of solvent (bacteriostatic water, PBS, etc.) added
Required only for molar concentration (µM)
Concentration (mg/mL) = peptide mass (mg) ÷ solvent volume (mL). This gives the mass concentration of your reconstituted peptide solution.
µg/mL = mg/mL × 1000. Used when protocols call for microgram-level concentrations.
Molar concentration (µM) = (µg/mL ÷ molecular weight in g/mol) × 1000. Required when a protocol specifies a molar concentration for receptor-binding or enzyme assays.
Research Use Only
This calculator is provided as a laboratory tool for qualified researchers preparing peptides for in vitro experiments. All peptides referenced are sold strictly for research purposes — not for human consumption, diagnosis, or treatment.
Browse our catalog of laboratory-grade peptides — each backed by batch-specific Certificates of Analysis.
View Research PeptidesWhy Concentration Accuracy Matters
Accurate reconstitution is essential for reproducible in vitro research. Always use calibrated pipettes, record the exact solvent volume, and verify the peptide mass from the vial label or Certificate of Analysis.